Specific antigen of tumor cell transformed by DNA extracted from SV-40 virus.

In the immunofluorescent study it has been revealed that rabbit sera immunized with transformed cells induced by SV-40 DNA, produce circulating antibody capable of re:lcting with intranuclear antigens synthesized by SV-40 complyte virus transforming process, In addition, the result confirmed that SV-40 DNA replicates DNA-containing viruses in the host cell and that also the genome coding for the synthesis of SV-40 tumor antigen is resposible for viral DNA. ∗PMID: 4294709 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 21, 51-57 (1967) SPECIFIC ANTIGEN OF TUMOR CELL TRANSFORMED BY DNA EXTRACTED FROM SV-40 VIRUS Tetsuo KIMOTO, Morio SHIGEHISA, Kenji HAYASHI, Shigeru OKADA, Koichi SOGABE and James T. GRACE* Department of Pathology, Okayama University Medical School, Okayama, Japan (Head of the Department: Prof. S. Sen0) Received for publication, January 5, 1967 It has recently been demonstrated that besides viral antigen the monkey kidney cells in culture infected with SV-40 virus synthesize the specific non-viral antigen as well as in the case of the SV-40 induced hamster tumor cell. Later on, it has been elucidated that this specific antigen is also present in the virus-affected cells of human (SABIN, SHEIN, KOCH and ENDERS), rabbit, mouse and porcine origin (BLACK and ROWE) in culture that have attained the morphologic transformation by the infection with SV-40 virus. These works have been made mainly by employing the immunofluorescent method by using the antibodies from the serum of the tumor bearing hamster induced by injecting SV-40 virus. The similar hamster tumors have been induced by injecting DNA isolated from the cultured cells with SV-40 virus • To obtain a definite evidence whether or not the tumor induced by the viral DNA is identical with the one induced by the virus itself, we have attempted to reveal the existence of the specific antigen similar to those produced by the viral infection. The cell transformation or the cell dedifferentiation have also been studied morphologically. MATERIALS AND METHODS NA-FS cell line : The cell line was originally derived from a hamster tumor (fibrosarcoma) induced by DNA extracted from SV-40 virus. It has undergone 43 passages in tissue culture during 14 months. Attempt to isolate infectious virus from the cells as well as from all transplanted tumors have been unsuccessful. The cells (Photo 1) are grown in 16-ounce bottles in fluid nutrient composed of McCoy's 5 A medium supplemented with 10 percent fetal calf serum. (100 U. penicillin and 100/-tg streptomycin are added per m!.) * Director of Roswell Park Memorial Institute, Buffalo, New York, U. S. A.